Purification and analysis of DNA from low DNA content food samples

Yayla M. E. A., Dogan C., Sarioglan S.

JOURNAL OF CONSUMER PROTECTION AND FOOD SAFETY, cilt.14, sa.2, ss.147-155, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 2
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1007/s00003-018-1192-7
  • Dergi Adı: JOURNAL OF CONSUMER PROTECTION AND FOOD SAFETY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.147-155
  • Anahtar Kelimeler: DNA extraction, Zeolite Y, Low DNA content food, Real-time PCR, REAL-TIME PCR, EXTRACTION METHODS, GENOMIC DNA, ZEOLITE-Y, ADSORPTION, QUANTIFICATION, INHIBITION, PROTEINS, MATRICES, ACID
  • Marmara Üniversitesi Adresli: Hayır

Özet

Recently, molecular biology techniques are widely used in food analysis; yet, the complexity of food matrices causes difficulty in purification and analysis of the analyte. The aim of this study was to introduce a new material, zeolite Y, that is able to isolate DNA even from low DNA content food samples; and, to demonstrate the integrity of isolated DNA via real-time polymerase chain reaction. Although the purity of the isolated DNA has to be improved with further work, Zeolite Y was shown to have a potential to be effectively used in DNA isolation for food analysis especially for low DNA content food samples.