Bioactivities of Achillea phrygia and Bupleurum croceum based on the composition of phenolic compounds: In vitro and in silico approaches

Zengin G., EMRE G., Mollica A., Haznedaroglu M. Z., DOĞAN A., Aktumsek A.

FOOD AND CHEMICAL TOXICOLOGY, cilt.107, ss.597-608, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 107
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.fct.2017.03.037
  • Dergi Adı: FOOD AND CHEMICAL TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.597-608
  • Anahtar Kelimeler: Achiella phrygia, Bupleurum croceum, Bioactive compounds, Natural agents, Turkey, ANTIOXIDANT ACTIVITY, MILLEFOLIUM L., BIOLOGICAL-ACTIVITIES, CHLOROGENIC ACIDS, CRYSTAL-STRUCTURE, POTENTIAL SOURCES, ASPHODELINE TAXA, NATURAL AGENTS, ALPHA-AMYLASE, ESSENTIAL OIL
  • Marmara Üniversitesi Adresli: Evet

Özet

This study presents the effects of the Achillea phrygia Boiss. et Bal. and Bupleurum croceum Fenzl. extracts obtained by different solvents (ethyl acetate, methanol and water) on selected enzyme inhibitory effects and antioxidant ability with screening bioactive compounds. Total and individual bioactive compounds were detected by spectrophotometric and HPLC-DAD techniques. Antioxidant abilities were evaluated by different methods including free radical scavenging (ABTS and DPPH), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating. Enzyme inhibitory effects were tested against cholinesterases, tyrosinase, amylase, glucosidase and lipase. Total phenolic contents were ranged from 20.52 mgGAE/g extract (B. croceum methanol extract) to 41.13 mgGAE/g extract (A. phrygia methanol extract). Generally, methanol and water extracts showed the strongest antioxidant abilities, while the ethyl acetate extracts had the most promising enzyme inhibitory effects. HPLC analysis revealed the abundance of some phenolics including rutin, quercetin, sinapic acid and chlorogenic acid, respectively. These components were also assessed using molecular modelling with the aim to study their docking properties on a set of six enzymes used in this study. Overall, these species could be suggested as valuable sources of natural-bioactive agents for developing new functional, pharmacological and health promoting ingredients. (C) 2017 Elsevier Ltd. All rights reserved.