Single pot in situ aqueous derivatization and subsequent determination of streptomycin and dihydrostreptomycin residues in honey by means of mass spectrometry

Akyildiz I. E., Acar S., KÖK YETİMOĞLU E., Raday S., Erdem O., Uzunoner D., ...Daha Fazla

FOOD CHEMISTRY, cilt.405, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 405
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1016/j.foodchem.2022.134826
  • Dergi Adı: FOOD CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Honey, Aminoglycosides, Residue, In situ derivatization, UHPLC-MS, MS, LIQUID-CHROMATOGRAPHY, AMINOGLYCOSIDE ANTIBIOTICS, PRECOLUMN DERIVATIZATION, ANIMAL FEEDS, RAT PLASMA, MILK, FLUORESCENCE, PHASE, REAGENTS, ELISA
  • Marmara Üniversitesi Adresli: Evet

Özet

Streptomycin (STR) and dihydrostreptomycin (DSTR) are the typically encountered aminoglycoside (AMG) residues in honey. For AMG analysis, studies in literature involve impractical and expensive applications such as ion-pairing chromatography, immunoassays, pre and post column derivatizations, or SPE approaches. Pre -treatments of these methods are toilsome and costly. Herein, one-pot, aqueous in-situ derivatization method was presented as a superior protocol. Time and cost-efficient UHPLC-MS/MS method has been developed, and practical sample preparation was introduced. Satisfactory results were reported in method verification studies. The mean recovery values were 102.6% for STR and 101.3% for DSTR. Average values between 1.5% and 9.9% RSDs were found at intra and inter-day precisions. CC alpha (5.7 and 5.8 mu g/kg) and CC beta (6.2 and 6.4 mu g/kg) values were calculated for STR and DSTR respectively. AMG residues were found in 29 out of 110 analyzed samples using validated method. Described novelty enabled comprehensive analysis in an inexpensive and straightfor-ward manner.