Fabrication of Gentamicin Sulfate-Loaded 3D-Printed Polyvinyl Alcohol/Sodium Alginate/Gelatin-Methacryloyl Hybrid Scaffolds for Skin Tissue Replacement

Izgordu M. S., Ayran M., ULAĞ S., Yildirim R., Bulut B., ŞAHİN A., ...Daha Fazla

MACROMOLECULAR MATERIALS AND ENGINEERING, cilt.308, sa.12, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 308 Sayı: 12
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1002/mame.202300151
  • Dergi Adı: MACROMOLECULAR MATERIALS AND ENGINEERING
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Applied Science & Technology Source, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, INSPEC, Metadex, Civil Engineering Abstracts
  • Anahtar Kelimeler: 3D printing, alginate, gelatin, PVA, scaffold, skin tissue
  • Marmara Üniversitesi Adresli: Evet

Özet

3D-printed scaffolds can better mimic the function of human skin, both biologically and mechanically. Within the scope of this study, the effect of the addition of different amounts (10, 15, 20 mg) of gentamicin sulfate (GS) to a 10 mL solution of natural and synthetic polymers is investigated. Sodium alginate (SA), gelatin-methacryloyl (GelMA), and polyvinyl alcohol (PVA) are chosen as bioactive materials. The surface morphology and pore structures are visualized by scanning electron microscopy (SEM). According to the results, it is observed that the pore sizes of all scaffolds are smaller than 270 µm, the lowest value (130 µm) is obtained in the scaffold loaded with 15 mg GS, and it also has the highest tensile strength value (12.5 ± 7.6 MPa). Similarly, it is observed that the tensile strength (9.7 ± 4.5 MPa) is high in scaffold loaded with 20 mg GS. The biocompatibility test is performed with fibroblast cells, and the results show that the scaffolds are biocompatible with cells. The antibacterial test is carried out against the S.aureous and E. coli and the results indicate that all GS-loaded scaffolds demonstrate antibacterial activity.