NUTRITION RESEARCH, vol.150, pp.62-71, 2026 (SCI-Expanded)
Bioelectrical impedance analysis (BIA) is a widely used, noninvasive method for assess-
ing body composition. However, its sensitivity to acute changes in water consumption and
coffee intake raises concerns about measurement reliability. This study hypothesized that
the short-term consumption of 200 mL caffeinated or decaffeinated coffee would result in
measurable changes in BIA-derived body composition parameters compared to water in-
take. Twenty-seven healthy female participants (aged 18-35 years; body mass index 18.5-
29.9kg/m²)wererandomlyassignedtooneofthreegroups:caffeinatedcoffee,decaffeinated
coffee, or water (n = 9 per group). After all groups consumed 200 mL of the assigned bever-
age, BIA measurements were performed at baseline, 30, 60, 90, and 120 minutes using the
InBody 270 analyzer. Nonparametric tests (Kruskal–Wallis, Friedman) and Quade ANCOVA
were applied to assess between- and within-group differences, adjusting for baseline val-
ues. Significant within-group changes were observed in body weight and body mass index
across all groups (P < .05). Between-group differences emerged in fat mass, fat percentage,
and body mass index at multiple time points (P < .05); however, none remained significant
after adjusting for baseline using Quade ANCOVA (all P > .05). Notably, several parameters
showedmediumtolargeeffectsizes,despitenonsignificantP values.Undercontrolledcon-
ditions, moderate caffeine intake (∼95 to 100 mg) or 200 mL fluid volume does not induce
significant short-term alterations in BIA-derived body composition in healthy women. Vi-
sual trends suggest transient fluctuations within the first 60 minutes, though these were
not clinically meaningful.