Whey Protein Concentrate Ameliorates the Methotrexate-Induced Liver and Kidney Damage

Tufan E., Sivas G. G., Gökmen B. G., Karaoǧlu S. Y., DURSUN E., Ak E., ...Daha Fazla

British Journal of Nutrition, cilt.130, sa.10, ss.1704-1711, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 130 Sayı: 10
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1017/s0007114523000752
  • Dergi Adı: British Journal of Nutrition
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, CINAHL, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1704-1711
  • Anahtar Kelimeler: Methotrexate, Whey proteins, Oxidative stress, Liver, Kidney, RENAL INJURY, ACID, THYMOQUINONE, MUCOSITIS, TOXICITY, BLOOD, RATS
  • Marmara Üniversitesi Adresli: Evet

Özet

Methotrexate (MTX) is a cytotoxic immunosuppressant that is widely used in the treatment of tumours, rheumatoid arthritis, and psoriasis. This study aims to evaluate the effects of whey proteins on MTX-induced liver and kidney damage by focusing on oxidant-antioxidant systems and eating habits. The study was conducted in four groups of 30 Sprague-Dawley rats (control, control+whey protein concentrate (WPC), methotrexate, methotrexate+WPC. A single dose of 20 mg/kg MTX was administered intraperitoneally to the MTX groups. Control and MTX groups were given 2 g/kg WPC by oral gavage every day for ten days. At the end of day 10, blood samples were drawn and liver and kidney tissues were removed. MTX administration increased the lipid peroxidation level and decreased glutathione level, superoxide dismutase and glutathione-S-transferase activities in the liver and kidney. Administration of WPC significantly reduced the damage caused by MTX in the liver and kidney. While a decrease in serum urea level and an increase in serum creatinine level were detected in the MTX group, WPC administration reversed these results up to control group levels. Administration of WPC to the MTX group significantly reversed the histopathological damage scores of the liver and kidney. Whey protein concentrate administration ameliorated the MTX-induced oxidative damage in the liver and kidney tissues due to its antioxidant properties. Liver and kidney damage can be prevented by using whey proteins as a nutraceutical in MTX therapy. In conclusion, whey proteins demonstrated a protective effect against MTX-induced liver and kidney damage.