Modelling and simulation of a transketolase mediated reaction: Sensitivity analysis of kinetic parameters

Sayar N. A. , Chen B. H. , Lye G. J. , Woodley J. M.

BIOCHEMICAL ENGINEERING JOURNAL, cilt.47, ss.1-9, 2009 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 47
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.bej.2009.02.011
  • Sayfa Sayıları: ss.1-9


In this paper we have used a proposed mathematical model, describing the carbon-carbon bond format ion reaction between beta-hydroxypyruvate and glycolaldehyde to synthesise L-erythrulose, catalysed by the enzyme transketolase, for the analysis of the sensitivity of the process to its kinetic parameters. The model was validated with experimental data. As a conclusion, kinetic parameters with a possible positive impact on reaction performance were identified and assessed in relation to operating conditions. This resulted in the identification of suitable catalyst and process development targets. (C) 2009 Elsevier B.V. All rights reserved.