Akademik Veri Yönetim Sistemi
Southern Clinics of Istanbul Eurasia, cilt.33, sa.1, ss.41-45, 2022 (Hakemli Dergi)
Objective: Nowadays, the treatment of infections caused by hospital-acquired resistant
bacteria has become very difficult. It is known that hospital infections can be limited and kept
to a minimum with the use of appropriate sterilization–disinfection methods. In our study,
we aimed to investigate the inhibition effect of ozone—which is low cost, has a nontoxic
effect on humans, and does not leave chemical residues and wastes—on resistant microorganisms that cause nosocomial infections.
Methods: In this study, 80 strains of bacteria with various resistance patterns and isolated
as a causative agent of nosocomial infection were included. Ten strains of each bacterium—
MRSA, VRE, MDR Pseudomonas aeruginosa, ESBL (+) Klebsiella pneumoniae, carbapenemase (+) K. pneumoniae, colistin-resistant K. pneumoniae, colistin-resistant Acinetobacter
baumannii complex, and colistin sensitive A. baumannii complex—were used. One liter of
sterile distilled water (DW) was saturated with ozone for 1 h. A quantity of 0.1 mL of bacterial suspension was added onto 9.9 mL ozonated DW (final bacterial concentration 106
cfu/mL). From the suspensions kept at room temperature, samples were inoculated as a
count plate on sheep blood agar with a 10 µL calibrated loop at 10 and 30 min. After 24 h
of incubation, the number of growing colonies was calculated by evaluating the Petri dishes.
Results: The bacterial inhibition rates of ozonated water at 10 and 30 min exposure times
were detected as 97.29–100% for Gram-positive nosocomial-resistant pathogens and 94.76–
99.99% for Gram-negative nosocomial-resistant pathogens, respectively.
Conclusion: It has been determined that ozonated water can provide a very high antibacterial effect in vitro at a very low cost. In other studies, the antiviral activity of ozone, including
SARS-CoV-2, has also been shown. The data we obtained suggest that ozone can be used
in various disinfection–sterilization processes in hospitals. We believe that a cost-effective
solution can be produced by supporting such studies with clinical research.