ANTI-CANCER EFFECT OF TRIAZOLE-THIOETHER HYBRID MOLECULE DERIVED FROM S-NAPROXEN (SGK669) COMPOUND ON T47D AND MDA-MB-453 HUMAN BREAST CANCER CELL LINES


Çuhadar O., Mega Tiber P. , Orun O. , Birgül K., Küçükgüzel Ş. G.

Cell Death: Degeneration and Regeneration Symposium, İstanbul, Türkiye, 3 - 05 Ekim 2019, ss.58-59

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: İstanbul
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.58-59

Özet

Cancer is an important problem of modern medicine as the most common cause of death after cardiovascular diseases (1). In recent years, significant toxicity and drug resistance have emerged with anticancer treatments. The search for drugs that can induce apoptosis in cancer cell lines and delay tumor growth has taken its place as one of the most effective treatment methods. Naproxen, known to have nonsteroidal antiinflammatory activity, is used in human breast cancer (2), bladder cancer (3), colorectal adenocarcinoma (CaCo2), hepatocellular carcinoma (HepG2), epitheloid cervical carcinoma (Hela), Lung carin (A5W9). ), mammary gland carcinoma and epidermoid larynx carcinoma (Hep2) (4) cells have been reported to have anticancer activity. In this study, we aimed to determine the anticancer and biological effects of triazole-thioether hybrid molecule derived from S- Naproxen, SGK669, which was synthesized in Marmara University Faculty of Pharmacy Department of Pharmaceutical Chemistry on different breast cancer cell lines T47D, MDA-MB-453. T47D and MDA-MB-453 cell lines were cultured in RPMI and DMEM medium in 5% CO2 at 37°C with different concentrations of SGK669 (10-25-50-75-100μM) for 24 and 48 hours. Growth inhibition of the compound was evaluated by the MTT test. The IC50 value 6 μM ,15 μM and13 μM,15 μM following 24 and 48h for T47D cell line and MDA-MB-453 cell line, respectively. The apoptotic effects of different concentrations of compound (SGK 669) on T47D and MDA-MB-453 cells were determined by the JC1-Mitochondrial Membrane Potential kit and the Tali Annexin-PI Apoptosis kit assays. Our results show that SGK669 inhibits cell viability and apoptosis was induced by SGK669 in a concentration-dependent manner.

Keywords: Naproxen, Breast Cancer, T47D, MDA-MB-453, Cytotoxicity, Apoptosis