Akademik Veri Yönetim Sistemi
5th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2022) N, Ankara, Türkiye, 23 - 25 Kasım 2022, ss.1715
Abstract
Cancer is the most common and deadly disease in the world after cardiovascular diseases. Human prostate cancer ranks
second in incidence worldwide after lung cancer in men. Anticancer, anti-inflammatory, antioxidant, antimicrobial,
antiviral and antiobesity effects of some plants are known. The genus Lamium is a herbaceous species in the Lamiaceae
family. Antioxidant, antibacterial, antimicrobial, anti-inflammatory, cytotoxicity and antityrosinase activities of the genus
Lamium have been shown before. In this study, the anticancer effect of Lamium purpureum, a species belonging to the
genus Lamium, was studied in LNCaP androgen-dependent human prostate cancer cell lines. Extracts derived from n-hexane (SSH), aqueous ethanol (SSSE), chloroform (SSK), ethyl acetate (SSEA), maceration ethanol (SSME) prepared
from Lamium purpureum plant were used. The cytotoxic effect of the extracts was examined with the CCK-8 kit after 24
and 48 hours of incubation. IC50 values were calculated using the results obtained. Accordingly, the IC50 values for SSH,
SSSE, SSK, SSEA, SSME in the LNCaP cell line were 20.33, 86.66, 78.07, 70.41, 27.18 ug/ml for 24 hours; 19.93, 48.81,
35.05, 56.92, 15.35 ug/ml for 48 hours, respectively. The JC-1 mitochondrial membrane potential test was used to
determine possible depolarization in the mitochondrial membrane potential. As a result of the 48-hour study with SSH
and SSK, no depolarization tendency was observed in the mitochondrial membrane potential. These preliminary results
suggest that the extracts suppress cell proliferation by showing cytotoxic effects, but may not stimulate the intrinsic
apoptotic pathway. Further studies will be carried out to support the results.
This study was supported by Marmara University Scientifical Research Projects Commission with project number TYL-2022-10417.
Keywords: Lamium purpureum, LNCaP, prostate cancer, apoptosis, anticancer