PEG-based hydrogel-coated test strip for on-site urea determination


Alev-Tuzuner B., Beyler-Cigil A., Kahraman M. V. , Yarat A.

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, vol.68, pp.597-606, 2019 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 68
  • Publication Date: 2019
  • Doi Number: 10.1080/00914037.2018.1482460
  • Title of Journal : INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
  • Page Numbers: pp.597-606
  • Keywords: Colorimetric biosensor, PEG-based hydrogel, urea test strip, urease, IMMOBILIZATION, BIOSENSOR, BLOOD, MICROPARTICLES, ELECTRODE, NITROGEN, ARRAYS

Abstract

The urea test strip was prepared using polyethyleneglycol based hydrogel, urease and commercial pH-indicator paper. The swelling behavior of hydrogel indicated the ability of the urea to penetrate into the matrix and allowing the test strip to respond in a short time. The color formed on the strip was distinguished by naked eyes in one minute at room temperature. The linear range for urea was 20-200 mg/dL. The assays have good reproducibility. Relative standard deviation values were found within the range of 3.37-12.95% and 1.68-9.54% for intra- and inter-day assays, respectively.