Akademik Veri Yönetim Sistemi
Clinical and Experimental Health Sciences, cilt.15, sa.3, ss.690-694, 2025 (ESCI, TRDizin)
Objective: The aim of this research is to evaluate the tissue-level expression of LATS1, YAP1, PI3KCG, and WWTR1 genes, which are key components of the Hippo-YAP/TAZ signaling pathway, in relation to glioma development. Methods: This research included tissue samples collected from 30 patients aged between 18 and 80 years who underwent neurosurgical resection at the institution’s affiliated hospital with a diagnosis of glioma. Tumor tissue samples were processed for total RNA isolation. Complementary DNA (cDNA) synthesis was subsequently performed, followed by quantitative polymerase chain reaction (qPCR) analysis to assess the relative expression levels of the selected genes. All procedures were conducted in compliance with standardized molecular protocols, and data were statistically analyzed using appropriate methods to determine expression differences. Results: Among the genes analyzed, YAP1 demonstrated a statistically significant 2.6-fold downregulation in glioma tissues compared to adjacent non-tumoral tissues (p = 0.03). Expression changes in other genes were observed, but did not reach statistical significance within the scope of this study. Conclusion: Our findings suggest that YAP1 may play a critical role in glioma pathogenesis. The observed downregulation indicates a potential dysregulation of the Hippo-YAP/TAZ signaling pathway in tumor development. These results underscore the importance of further investigating YAP1 and related signaling components as potential therapeutic targets in glioma and other central nervous system tumors. Future studies with larger patient cohorts and functional analyses are warranted to validate these preliminary findings.