Mycophenolate mofetil attenuates uterine ischaemia/reperfusion injury in a rat model

Ersoy G. S., Eken M. K., ÇEVİK Ö., Cilingir O. T., Tal R.

REPRODUCTIVE BIOMEDICINE ONLINE, vol.34, no.2, pp.115-123, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 2
  • Publication Date: 2017
  • Doi Number: 10.1016/j.rbmo.2016.11.007
  • Journal Name: REPRODUCTIVE BIOMEDICINE ONLINE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.115-123
  • Keywords: mycophenolate mofetil, oxygen species-free radicals, rat, uterine ischaemia-reperfusion, uterine transplantation, ISCHEMIA-REPERFUSION INJURY, UTERUS TRANSPLANTATION, ISCHEMIA/REPERFUSION INJURY, LIVER-TRANSPLANTATION, OXIDATIVE STRESS, KIDNEY, ADHESION, DONOR, ALLOTRANSPLANTATION, TACROLIMUS
  • Marmara University Affiliated: Yes

Abstract

This study evaluated the effect of mycophenolate mofetil (MMF) on uterine tissue preservation following ischaemia/reperfusion (I/R) injury. Uterine I/R injury was induced in rats by clamping the lower abdominal aorta and ovarian arteries for 30 min. Group I/R + V (n = 7) received vehicle alone while Group I/R + M (n = 7) received 20 mg/kg/day MMF. Control groups underwent sham surgery and received vehicle (Group C) or 20 mg/kg/day MMF (Group M) (n = 7 for both). Four hours after detorsion, uterine tissue 8-hydroxy-2'-deoxyguanosine (8-OHdG), glutathione, malondialdehyde (MDA), myeloperoxidase (MPO), superoxide dismutase (SOD) and serum ischaemia modified albumin (IMA) concentrations were measured. Histopathological analyses were performed. The I/R + M group showed significant reduction in serum IMA and uterine tissue 8-OHdG, MDA and MPO and significant increase in SOD concentrations compared with the I/R + V group, indicating a protective effect against I/R oxidative damage (P = 0.009, P = 0.006, P = 0.002, P = 0.003 and P = 0.009, respectively). Histopathological evaluation revealed MMF treatment resulted in significantly less tissue and cellular damage and apoptosis compared with the I/R + V group. These results indicate MMF is effective in attenuating uterine tissue damage and preventing apoptosis following uterine I/R injury, probably via anti-inflammatory and anti-oxidative action. (C) 2016 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.