Akademik Veri Yönetim Sistemi
European Congress of Clinical Microbiology and Infectious Diseases, Paris, Fransa, 18 - 21 Nisan 2020, ss.2775
Background: Aminoglycosides (Ags) are often used in
combination with other antibiotics and provide a valuable therapeutic option
for treating multidrug resistant bacterial infections. Aminoglycoside
resistance (AgR) due to 16S ribosomal methyltransferases (RMTs) seems to emerge
as real threat that limit the use of these antibiotics for salvage therapy. In
this study, we aimed to investigate genetic determinants of RMTs together with
aminoglycoside modification enzymes (AgMEs) in invasive Klebsiella pneumoniae
and Escherichia coli. Materials/methods: Fifty K. pneumoniae and 49 E. coli,
isolated throughout the year 2016 and exhibited resistance to at least amikacin
or gentamicin in routine susceptibility testing (VITEK® 2 Compact, BioMérieux,
France) were included in the study. Amikacin, gentamicin, tobramycin, and
netilmicin MICs of the isolates were determined by standard broth microdilution
method (BMD) in the study isolates. Genetic determinants of RMTs (armA, rmtA,
rmtB, rmtC, rmtD, rmtE, rmtF, rmtG, rmtH, npmA) and AgMEs [aac(3)-II, aac(3)-I,
aac(6’)-Ib, ant(2’’)-I] were investigated by PCR. Results: Twelve of 50 (24%)
K. pneumoniae isolates exhibited high level resistance (>256 mg/L) to all
Ags tested in BMD. High level aminoglycoside resistance was not detected in any
of the E. coli isolates. rmtC was the most common (13/50) RMT gene followed by
armA (2/50) in K. pnuemoniae (Table 1). aac(3)-II was found together with
aac(6’)-Ib in 64% of K. pneumoniae and 32.7% of E. coli isolates. Conclusions:
rmtC encoded high level aminoglycoside resistance seems to be a threat in K.
pneumoniae in our hospital, especially considering high risk of multidrug
resistance in such isolates.