Akademik Veri Yönetim Sistemi
Turkish Society of Physiological Sciences, 49th Turkish Physiology Congress, 6–9 November 2024, Izmir, Türkiye Volume 241, Issue S733, PC-01, İzmir, Türkiye, 6 - 09 Kasım 2024, ss.60, (Özet Bildiri)
PC-01
Protective Effect of Aerobic Exercise Against Temozolomide-
induced Hepatotoxicity in Rats with an Experimental
Glioblastoma Model
Aslı Nur Bahar1, Leman Arslan Arıtürk1, Ikbal Tan2, Nisanur Duman2
,Nurhan Beyza Düzgün2, Sakine Beyza Üstün2, Zülal Sorkun2, Banu
Taktak Karaca3, Dila Şener Akçora4, Özgür Kasımay1
1Marmara University, Faculty of Medicine, Department of
Physiology, Istanbul, Türkiye
2Marmara University, School of Medicine, 3rd Year Medical
Students, İstanbu, Türkiyel
3Marmara University School of Medicine, Department of
Immunology, İstanbul, Türkiye
4Marmara University, School of Medicine, Department of Histology
and Embryology, İstanbul, Türkiye
AIM: Glioblastoma (GBM) is a common and aggressive brain
tumour with a median survival of 12-15 months. Temozolomide
(TMZ) is the main chemotherapeutic agent used in the treatment
of GBM. Liver injury has been reported in patients treated with
TMZ. In this study, we aimed to investigate the protective role of
moderate aerobic exercise against TMZ-induced hepatotoxicity in
rats with glioblastoma.
METHODS: A total of 50 three-month-old Sprague-Dawley rats
were used to form 5 groups; sham-operated (S), glioblastoma
(GBM), glioblastoma+temozolamide (GBM+T), glioblastoma+
exercise (GBM+E) and glioblastoma+temozolamide+exercise
(GBM+T+E). A moderate-intensity exercise protocol at 50-60% of
the rats' VO2max was applied to the exercise groups for 30
min/day for a total of 8 weeks. The tumour model was established
by intracerebroventricular injection of 1x106 glioma cells into the
rats by the stereotaxic method. Subsequently, the chemotherapy
groups were given 10 mg/kg TMZ by oral gavage. After
sacrification, liver tissues were isolated and the levels of MDA, GSH
and catalase, SOD and MPO in tissue; ALT and AST in serum were
measured by spectrophotometer. Histopathological scoring was
performed. Statistical evaluation was performed by one-way
ANOVA and post hoc LSD.
RESULTS: GBM administration increased MDA levels in liver tissue.
However, MDA levels decreased in the exercise groups (GBM+E
and GBM+TMZ+E) (p<0.05). There was no significant difference in
MDA levels between the GBM and GBM+T groups. The antioxidant
catalase activity increased with exercise and/or TMZ
administration compared to the control group (p<0.01). GSH levels
increased in the GBM, GBM+E and GBM+TMZ+E groups compared
to the control group (p<0.05). TMZ treatment increased plasma
ALT and AST, whereas exercise decreased them (p<0.05). The
histopathological score increased in G and GT groups and
decreased in GE and GTE groups (p<0.05).
CONCLUSIONS: The results of the study showed that moderate
aerobic exercise was beneficial in reducing the detrimental effects
of temozolomide on the liver. (supported by TUBITAK-2209-A).
Keywords: Glioblastoma, Temozolomide, Exercise, Hepatotoxicity