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EUROPEAN JOURNAL OF HUMAN GENETICS, vol.1, no.32, pp.349-795, 2024 (SCI-Expanded, Scopus)
Chronic renal insufficiency (CRI) is a public problem effecting cardiovascular well being and life expectancy. Vascular calcification (VC, is mineralization of vascular tree with the loss of arterial and venous flexibility. As normal end result of aging process, VC causes vascular stiffness increasing morbidity and mortality. Material and Methods: Herein we investigated RUNX2 and IL6/STAT3 signalling pathways through hsa-miR-30a-5p, hsa-miR223-3p and downstream members of these pathways using RNA PCR technique. From the waste vascular tissue of 37 patients collected during renal transplantation operation expression levels of target miRNA and genes were analyzed with qRT-PCR at the Biorad Real time PCR platform. Results: There were 8 females and 29 male patients with an average age of 36+/-14 years. For normalization of expression levels hsa-mir-145-5p was used as intermal control which is expressed atvascular tissue at physiological condition. Conclusion: We have detected that Hsa-miR-223-3p and Hsa- miR-30a-5p expressions were increased in vascular tissue of chronic renal failure patients effecting osteoblastic differentiation at the vascular tissue. Conflict of Interest: None declared