Determination of finasteride in the tablet form by liquid chromatography and its analytical method validation


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Demircan Demir H., Cucu A. , Sakarya S.

ANALYTICA CHIMICA ACTA, vol.557, pp.252-255, 2006 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 557
  • Publication Date: 2006
  • Doi Number: 10.1016/j.aca.2005.10.033
  • Title of Journal : ANALYTICA CHIMICA ACTA
  • Page Numbers: pp.252-255
  • Keywords: finasteride, Dilaprost (R), validation, RAT, 5-ALPHA-REDUCTASE

Abstract

A quantitative method for finasteride by liquid chromatography (LC) with UV detector, was validated for its new tablet form Dilaprost (R). Analysis was performed using Nova Pak C-18 column at 60 degrees C. Detection was carried out at a wavelength of 210 nm. The best separation for finasteride peak was achieved by isocratic elution with the mobile phase water/acetonitrile/tetrahydrofuran (80/10/10, v/v/v) and flow rate of 2 mL min(-1). The sample volume injected into liquid chromatography system was 15 mu L. Analytical method validation tests were performed. (c) 2005 Elsevier B.V. All rights reserved.