Akademik Veri Yönetim Sistemi
Materials Today Communications, cilt.49, 2025 (SCI-Expanded, Scopus)
This study aimed to develop bioactive scaffolds by incorporating Mentha pulegium essential oil (MPEO) loaded in polylactic acid (PLA), a secondary metabolite known as B-ionone (BI), and a combination of both in nanoparticle (NP) form. Bioactive NPs were fabricated using a 17 % PLA solution via the electrospraying technique. Thorough characterization of the final 3D-printed scaffolds involved scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR) to assess their morphological and chemical attributes. Additionally, mechanical, thermal, swelling, and degradation behaviors were evaluated alongside other characteristics. The characterization of the scaffolds revealed their remarkable quality across various tested criteria. Antibacterial assessments against four bacterial strains and the analysis of encapsulated bioactive compound release kinetics from 17 % PLA scaffolds were conducted. PLA@B-Ionone-NP-coated scaffolds demonstrated significant biocompatibility and notable antibacterial efficacy, particularly against Bacillus subtilis (ZOI = 12 mm) and Pseudomonas aeruginosa (ZOI = 9 mm). However, optimization of individual bioactive molecule concentrations revealed that PLA@MPEO+BI-NPs did not exhibit synergistic antimicrobial effects against the tested bacterial strains. Notably, PLA@MPEO-NP scaffolds displayed exceptional biocompatibility, indicating promise in reducing bacterial infections and promoting wound healing. Water contact angle measurements demonstrated increased hydrophilicity following the application of bioactive nanoparticles. This latter not only improves exudate absorption but also enhances antibacterial biocompatibility. Unlike previous research, which mainly focused on incorporating EO-NPs or SM-NPs into nanofibers for skin regeneration, our method involves combining 3D printed porous scaffolds with electrosprayed NPs containing bioactive molecules such as essential oils or/and secondary metabolites, representing a never before reported methodology.