Fabrication and Characterisation of GelMA/Keratin and GelMA/KerMA Hybrid Tissue Scaffolds by 3D Bioprinting

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Akdag Z., Baykara D., Gündüz O., Ulağ S.

Applications of Chemistry in Nanosciences and Biomaterials Engineering NanoBioMat 2023 – Winter Edition, Bucuresti, Romanya, 22 - 24 Kasım 2023, ss.77

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Bucuresti
  • Basıldığı Ülke: Romanya
  • Sayfa Sayıları: ss.77
  • Marmara Üniversitesi Adresli: Evet

Özet

Skin diseases that cause pain, damage, and infection to the skin are often used in tissue engineering studies. While these conditions can be addressed with different medications and antibiotics, dressing the affected area alone may not suffice. As such, an intervention for wound care should be introduced as early as possible using a more efficient approach. Natural polymeric hydrogels with multifunctional properties are highly desirable for enhancing the healing process of chronic wounds. An optimal photo-crosslinked skin covering, Gelatin methacryloyl (GelMA) can mimic the structure of the extracellular matrix (ECM). Nonetheless, pure GelMA hydrogel exhibits insufficient mechanical strength. Its application as a hybrid enhances its efficacy. Keratin, the world's third most abundant polymer, is a robust and filamentous protein. Keratin is a crucial constituent of mammals, reptiles, and bird hair, feathers, wool, nails, and horns. Furthermore, keratin waste is a valuable, renewable raw material, and it is necessary to expand the utilization of waste wool in order to add to its worth. By performing high-yield keratin extraction from sheep wool, keratin methacrylate can be derived through chemical modification of keratin. In comparison to keratin, keratin methacrylate demonstrates superior biocompatibility and cell adhesion properties. In the present investigation, a tissue scaffold system was fabricated, which includes GelMA, keratin, and keratin methacrylate to serve as cell and growth factor carriers for skin disorder therapy. The GelMA/keratin and GelMA/keratin methacrylate scaffolds will be assessed by comparing their swelling rate, biodegradation behavior, physical properties (FT-IR), chemical properties (DSC), morphology (SEM), and mechanical properties (tensile). Overall, it is believed that GelMA/keratin and GelMA/keratin methacrylate tissue scaffolds have potential as multifunctional patches for healing skin injuries via skin tissue engineering.