In Vitro Investigation of The Effects of Some Antibiotics on Polymorphonuclear Leukocyte Functions and Oxidative Stress in Hemodialysis Patients


Garip R., Gürer Ü. , GÜRBÜZ B. , Dalkiliç E., PALANDUZ Ş., Aysuna F. N. , ...More

MARMARA PHARMACEUTICAL JOURNAL, vol.18, no.3, pp.139-146, 2014 (Journal Indexed in ESCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 18 Issue: 3
  • Publication Date: 2014
  • Doi Number: 10.12991/mpj.2014187241
  • Title of Journal : MARMARA PHARMACEUTICAL JOURNAL
  • Page Numbers: pp.139-146

Abstract

In our study we were investigated the in vitro effects of therapeutic concentrations of vancomycin (40 mu g/ml), daptomycin (60 mu g/ml), linezolid (19 mu g/ml), levofloxacin (7 mu g/ml), fusidic acid (36 mu g/ml) and meropenem (24 mu g/ml) on the malondialdehyde (MDA ) levels and polymorphonuclear leukocyte (PMN) functions (phagocytic and intracellular killing activity) in hemodialysis patients. PMNs were isolated from venous blood by ficoll-hypaque gradient centrifugation method. Phagocytic activity and intracellular killing activity were assayed by modified Alexander"s method. MDA level was detected by Beuge method which is as an indicator of lipid peroxidation. All results was evaluated by statisticaly. Vancomycin, daptomycin, linezolid, levofloxacin, fusidic acid and meropenem at therapeutic concentrations did not affect both PMN functions of HD patients before and after HD (p>0.05). It was observed that vancomycin, levofloxacin, fusidic acid and meropenem at therapeutic concentrations increased PMN"s MDA levels of HD patients before HD but decreased MDA levels after HD (p<0.05). Daptomycin and linezolid did not change PMN"s MDA levels of HD patients before and after HD (p>0.05).