Akademik Veri Yönetim Sistemi
11th Conseuro, Antalya, Türkiye, 21 - 23 Nisan 2022, ss.144
Investigation of Riboflavin as Biomimetic Mineralization on Demineralized Dentin by Optical Coherence Tomography
Pelin Kükey1, Ezgi Altuntaş1, Pınar Yılmaz Atalı1, Cafer Türkmen1, Betül Kargül2
1Marmara University, Faculty of Dentistry, Department of Restorative Dentistry, İstanbul, Turkey 2Marmara University, Faculty of Dentistry, Department of Pediatric Dentistry, İstanbul, Turkey
Aim: Optical coherence tomography (OCT) has considerable potential as a non-invasive imaging technique for carious lesions as it eliminates the need for the removal of the superficial layer and eliminates radiation. The aim of this study was to assess the biomimetic remineralization capacity of Riboflavin for demineralized dentin samples using spectral-domain optical coherence tomography (SD-OCT).
Materials and Methods: Twenty four dentin blocks were randomly divided into 6 groups as: 2% Riboflavin; Universal bonding agent (Optibond Universal, Kerr); Functional tricalcium phosphate (fTCP) (Clinpro TM 5000, 3M) ; Universal bonding agent+2% Riboflavin; fTCP + 2% Riboflavin; Remineralizing solution (Positive Control). The dentin samples were immersed in a demineralization solution maintained for 72 hours and remineralization agents were applied for 10 days. The optical depth of backscattered light of enamel was measured using Zeiss Cirrus HD SD-OCT 5000 (Carl Zeiss Meditec). SD-OCT analyzes were performed after demineralization and after remineralization procedures. The appropriate values that correspond to the visual boundary of dentin lesions were measured. Paired two-sample t-test was used to compare the application within the groups (p<0.05)
Results: At the baseline (after demineralization), there was no statistically significant difference in the optical lesion depths of the dentin blocks in the six groups (p=0.487). The reflectivity and the optical lesion depth decreased after remineralization in all groups. A significant decrease in the depth of optical lesion on the dentin surfaces in all groups was observed following the remineralization (p<0.05) except adhesive application (p>0.05)
Conclusion: Within the limitations of this study, the surface treatment with Riboflavin provided biomimetic dentin remineralization. Further investigations are needed on Riboflavin as a potential innovative bioactive material of dentine remineralization as minimal intervention which is an important concept in clinical restorative dentistry.
Keywords: biomimetic mineralization, riboflavin, tri-calcium phosphate, optical coherence tomography (OCT)