The in vivo metabolism of 5-(4-nitrophenyl)-4(2-phenylethyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione in rats


Oruc E., Kabasakal L. , Rollas S.

EUROPEAN JOURNAL OF DRUG METABOLISM AND PHARMACOKINETICS, vol.28, no.2, pp.113-118, 2003 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 2
  • Publication Date: 2003
  • Doi Number: 10.1007/bf03190498
  • Title of Journal : EUROPEAN JOURNAL OF DRUG METABOLISM AND PHARMACOKINETICS
  • Page Numbers: pp.113-118

Abstract

The purpose of this study was to investigate the in vivo metabolism of 5-(4-nitrophenyl)-4-(2-phenylethyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione. First its potential metabolites were synthesized and then the structures of the original compound were elucidated by UV, H-1-NMR and elemantary analysis. 40 mg dose was given intraperitoneally to rats. Blood samples were collected at 0, 0,5, 1, 2. 4. 6 12, 24, 48 and 72 hours after administration of substrate and blood was centrifuged to obtain plasma. The plasma were passed through a Sep-Pak C-18 cartridge. The samples were separated using HPLC on a reverse phase system. This study revealed reduction, N-acetylation and N-dealkylation as pathway of 5-(4-nitrophenyl)-4-(2-phenylethyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione metabolism.