Hydrogen Peroxide and Quercetin Induced Changes on Cell Viability, Apoptosis and Oxidative Stress in HepG2 Cells

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Yılmaz Göler A. M., Biçim G., Toprak K., Yılmaz B., Yalçın A. S., Milisav I.

Current Nutraceuticals, cilt.2, sa.1, ss.47-55, 2021 (Hakemli Dergi)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 2 Sayı: 1
  • Basım Tarihi: 2021
  • Doi Numarası: 10.2174/2665978601999200807160528
  • Dergi Adı: Current Nutraceuticals
  • Derginin Tarandığı İndeksler: Other Indexes
  • Sayfa Sayıları: ss.47-55
  • Marmara Üniversitesi Adresli: Evet

Özet

Background: Different cellular responses influence the progress of cancer. In this study, the effects of hydrogen peroxide and quercetin-induced changes on cell viability, apoptosis, and oxidative stress in human hepatocellular carcinoma (HepG2) cells were investigated.

Methods: The effects of hydrogen peroxide and quercetin on cell viability, cell cycle phases, and oxidative stress-related cellular changes were investigated. Cell viability was assessed by WST-1 assay. Apoptosis rate, cell cycle phase changes, and oxidative stress were measured by flow cytometry. Protein expressions of p21, p27, p53, NF-Kβ-p50, and proteasome activity were determined by Western blot and fluorometry, respectively.

Results: Hydrogen peroxide and quercetin treatment resulted in decreased cell viability and increased apoptosis in HepG2 cells. Proteasome activity was increased by hydrogen peroxide but decreased by quercetin treatment.

Conclusion: Both agents resulted in decreased p53 protein expression and increased cell death by different mechanisms regarding proteostasis and cell cycle phases.