The purpose of the study was to evaluate age and gender differences in physical self-concept of Turkish university students. The Physical Self-Perception Profile was administered to participants for assessing physical self-concept. Multivariate analysis of variance revealed a significant main effect for gender, but no significant main effect for year in school. There was also no year in school by gender interaction. Univariate analysis indicated significant gender differences in sport competence, physical condition, body attractiveness, and physical strength (favoring males except for body attractiveness).