Akademik Veri Yönetim Sistemi
Applications of Chemistry in Nanosciences and Biomaterials Engineering” NanoBioMat 2022 – Winter Edition, Bucuresti, Romanya, 24 - 26 Kasım 2022, ss.56-57
The skin tissue, which is the largest organ of the human body, acts as a barrier by controlling the entry of water, temperature, pressure, and foreign substances into the body. However, in some cases, this integrity may be disrupted, and skin injuries may occur. In addition to the surgical methods for the healing of these wounds, it is possible to produce modern-tech wound dressings that are suitable for the wound area by imitating extracellular matrices using biomaterials to accelerate the healing process. Keratin, being the third most abundant polymer in the world, is a hard and fibrous protein. It is the main component of hair, feathers, wool, nails, and horns of mammals, reptiles, and birds. In addition, keratin wastes are an important source of renewable raw materials and it is important to evaluate the waste wool to increase its value addition. In this study, keratin methacrylate (B-KerMA) was synthesized by chemically modifying keratin, which is extracted with a high yield from white sheep wool, using 2-mercaptoethanol. B-KerMA, which shows higher biocompatibility and cell adhesion properties compared to keratin, was used for the first time as the main wound dressing material together with PLA. While 10% PLA is used as the control group in nanofiber production, different proportions of B-KerMA such as 1%B-KerMA, 5%B-KerMA and 10%B-KerMA were added to it. The morphological, thermal, chemical, and mechanical properties of the dressings were evaluated using SEM, DSC, FT-IR, and tensile testing, respectively. In vitro cell culture experiments were performed on fibroblast cells using the XTT method. As a result of characterization and in vitro experiments, it has been shown that B-KerMA increases the biocompatibility and cell adhesion of PLA electrospun fibers. However, according to the results, the B-KerMA ratio should be a maximum of 10% due to the decrease in biocompatibility and cell adhesion.