An expedition across the Asian part of the Black Sea coast and national parks of Northern Turkey was organized in the summer of 2001 to investigate the presence of Borrelia burgdorferi sensu lato (s.l.), Lyme borrellosis agent, and Anaplasma phagocytophilum, human granulocytic ehrlichiosis agent, in wild mice. A total of 65 Apodemus./flavicollis, Apodemus sylvalicus, Microtus epiroticus, Crocidura suaveolens and Mus macedonicus, were captured. Two out of 22 Apodemus sylvaticus specimens were seropositive for B. afzelli by enzyme-linked immunosorbent assay as confirmed by Western blotting, however cultures of skin and bladder samples from all small mammals in Barbour-Stoenner-Kelly's medium-II remained negative for B. burgdorferi s.l. All sera tested were negative for Anaplasina phagocytophilum by indirect immunofluorescent assay. The prevalence of B. burgolorferi s.l. and Antiplasma phagocytophilum is low in wild mice of the Asian part of Northern Turkey.