Akademik Veri Yönetim Sistemi
Pediatrik Cerrahi Dergisi, cilt.17, sa.1, ss.35-40, 2003 (Scopus)
Aim: Ischemic preconditioning (IPC) was first demonstrated in the heart but this protective effect has been also recently described in the intestine. The aim of this study was to determine the effects of intestinal ischemic preconditioning on the morphology of intestine and bacterial translocation. Method: 24 male Wistar rats weighting 250-300 gr. were randomized into three groups. A control group of rats (n=8) were subjected to laparotomy. In an ischemic group (n=8), laparotomy was performed and the superior mesenteric artery was occluded by an atraumatic clamp for 30 min. In the preconditioned group (n=8), before the ischemia-reperfusion period (as in ischemic group), rats were subjected to initial 10 min of intestinal ischemia and 10 min of reperfusion. Twenty-four hours later, to evaluate whether the I/R induced intestinal injury and BT, tissue and blood samples were collected and liver, spleen, mesenteric lymph node specimens were obtained under sterile conditions for microbiological analysis. Samples of ileum were removed for histopathological evaluation. Results: In I/R group, the incidence of bacteria isolated from a mesenteric lymph nodes, spleen, liver and blood was significantly higher than other groups (p<0.05). IPC prevented I/R induced BT and significantly reduced the I/R induced intestinal injury (p<0.05). Increased iNOS expression observed on the ileal specimens of the I/R group was found to be prevented by IPC. Conclusion: Our data suggest IPC as a key factor that reduces bacterial translocation and iNOS activation in intestinal I/R. This is the first study showing that intestinal IPC blocks the cascade of events that causes bacterial translocation and intestinal injury which may lead to sepsis.