Spesific binding of LDL to purified GpIIb/IIIa and to isolated human platelets


Tetik S., Yardimci K., Uras F., Eksioglu-Demiralp E.

17th European and African Division Meeting of the International-Society-of-Haematology, Graz, Avusturya, 7 - 10 Eylül 2003, ss.205-208 identifier

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Basıldığı Şehir: Graz
  • Basıldığı Ülke: Avusturya
  • Sayfa Sayıları: ss.205-208

Özet

We have investigated the relationship between LDL, Fibrinogen (Fg), and GpIIb/IIIa. GpIIb/IIIa was purified from human platelets by affinity chromatography. Binding was assayed by flow cytometry. Polystyrene micro beads (Bangs Lab.) were coated with GpIIb/IIIa. Fluorescein isothiocyanate (FITC) labelled LDL, fibrinogen and BSA (negative control) were used as a ligands. Percent bound Fg-FITC or LDL-FITC to GpIIb/IIIa coated micro beads were found to be concentration dependent, reaching saturationAt saturation concentrations inhibited binding of g-FITC to GpIIb/IIIa coated micro beads dose dependently (30% max. Ynhibition). Percent bound LDL-FITC to GpIIb/IIIa coated micro beads was also inhibited by Fg (50% max. inhibition). Percent bound Fg-FITC and LDL-FITC to isolated platelets gave similar results with GpIIb/IIIa coated micro beads. Addition of purified GpIIb/IIIa caused inhibition of LDL-FITC biding to isolated platelets. GpIIb/IIIa, Fg-rcceptor on platelet is also LDL-receptor.