Bilayered laponite/alginate-poly(acrylamide) composite hydrogel for osteochondral injuries enhances macrophage polarization: An in vivo study

Saygili E., Saglam-Metiner P., Cakmak B., ALARÇİN E., Beceren G., Tulum P., ...Daha Fazla

BIOMATERIALS ADVANCES, cilt.134, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 134
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.msec.2022.112721
  • Dergi Adı: BIOMATERIALS ADVANCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Anahtar Kelimeler: Bilayered hydrogels, Osteochondral defect, Foreign body response, M1/M2 macrophage, Transcriptomic analysis, MESENCHYMAL STEM-CELLS, SCAFFOLDS, BIOCOMPATIBILITY, DIFFERENTIATION, OSTEOARTHRITIS, CYTOTOXICITY, ALGINATE, PATHWAY
  • Marmara Üniversitesi Adresli: Evet

Özet

Addressing osteochondral defects, the objective of current study was to synthesize bilayered hydrogel, where the cartilage layer was formed by alginate (Alg) polyacrylamide (PAAm) with and without the addition of TGF-beta 3 and bone layer by laponite XLS/Alg-PAAm and characterize by in vitro and in vivo experiments. Exceeding the mechanical strength of Alg-PAAm (32.95 +/- 1.23 kPa) and XLS based (317.5 +/- 21.72 kPa) hydrogels, XLS/Alg-PAAm hydrogel (469.7 +/- 6.1 kPa) activated macrophages towards M2 phenotype and stimulated the expression of anti-inflammatory factors. The addition of TGF-beta 3 accelerated transition of macrophage polarization, especially between day 4 and 7. The expression levels of MI-related genes such as CD80, iNOS and TNF-alpha decreased gradually after day 4, reaching lowest values at day 13, whereas the expression levels of M2-related genes, CD206, Argi and SFAT6 significantly increased promoting M2 macrophage polarization, which might be associated with accelerated bone repair. Moreover, bilayer structure exhibited a better cell viability as well as rcpainnent thorough the XIS contents. in vivo histological examinations verified the significant surface regularity and hyaline like tissue formation employment, along with synchronized degradation profile of the hydrogel with tissue healing at the end of 12 weeks. A mechanically durable, biocompatible and immunocompatiblc hydmgcl was formulated to be utilized in bone-cartilage engineering applications.