Aqueous garlic extract protects against sepsis induced toxicity in pulmonary and ileal tissues


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Özkan S., AKBAY T. , Sakarcan S., Günay E., ŞENER G.

Medical Science and Discovery, cilt.3, sa.4, ss.192, 2016 (Diğer Kurumların Hakemli Dergileri)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 3 Konu: 4
  • Basım Tarihi: 2016
  • Doi Numarası: 10.17546/msd.69902
  • Dergi Adı: Medical Science and Discovery
  • Sayfa Sayıları: ss.192

Özet


Objective: Based on the potent antioxidant effects of aqueous garlic extract (AGE), the present study was designed to characterize the potential of AGE to modify blood coagulation parameters as well as and pulmonary and ileal injury in septic rats. Sepsis was induced using the caecal ligation and perforation (CLP) method.

Material and Method:Twenty-four hours after sepsisinduction, rats were decapitated and trunk blood was collected for the measurement  of  platelet  counts,  fibrinogen,  prothrombin  time,  activated  partial  thromboplastin  time  (APTT)  and  d-dimer  levels. Then, pulmonary and ileal tissue samples were immediately obtained and stored at-70 °C for malondialdehyde (MDA), glutathione (GSH), myeloperoxidase (MPO) and superoxide dismutase (SOD) activity assays.

Results: Sepsis  was  associated  with  a  decrease  in  platelet  count  and  fibrinogen  and  an  increase  in  APTT  and  International  normalized  ratio.  It  also  caused  a  significant  decrease  in  GSH  levels  and  SOD  activity  in  both  pulmonary  and  ileal  tissue  samples. On the other hand, AGE treatment in rats with CLP caused significantly augmented the level of these antioxidants. As a result of CLP induction increased MPO activity and MDA levels and decreased thromboplastic activity were reversed with AGE treatment.

Conclusion:

 AGE treatment, through its antioxidant effects, protects against oxidative pulmonary and ileal injury and normalizes the impaired coagulation in sepsis.