Akademik Veri Yönetim Sistemi
FRESENIUS ENVIRONMENTAL BULLETIN, cilt.25, ss.3694-3699, 2016 (SCI-Expanded)
In this study, while doing research about the microbiological quality of 20 different well water samples which are used for drinking and domestic water by people in Kapakli (Ilgin- Konya/Turkey) between March-May 2015, it was aimed to determine the existence of motile Aeromonas in three well water samples which has the maximum total coliform number (>1100 MNP/100 mL). Yellow colonies which came into existence in consequence of incubation for 24-48 hours at 25-27 degrees C in GSP agar which is its selective medium were evaluated as suspicious Aeromonas sp. Colony, and they were put to oxidase test and gram staining. As a result of oxidase test, the water which was supposed to be positive (+) came out as oxidase negative (-). Therewith, 16S rRNA sequence analysis was done in order to classify suspicious colonies. In consequence of the sequence analysis, it was found out that the bacteria species which created yellow colony in GSP medium were Enterobacter ludwigii, Averyella dalhousiensis and Enterobacter aerogenes, respectively. In the comparison of sequence via BLAST it was determined that 99% of the sample 3 has nucleotide similarity to Enterobacter ludwigii, 99% of the sample 5 has nucleotide similarity to Averyella dalhousiensis, and 99% of the sample 10 has nucleotide similarity to Enterobacter aerogenes. According to the results, Enterobacter ludwigii and Averyella dalhousiensis were put on the record for the first time in Turkey via this study.