Bisphenol A reveals its obesogenic effects through disrupting glucose tolerance, oxidant-antioxidant balance, and modulating inflammatory cytokines and fibroblast growth factor in zebrafish

Beler M., Cansız D., Ünal İ., Üstündağ Ü. V., Dandin E., Ak E., ...Daha Fazla

TOXICOLOGY AND INDUSTRIAL HEALTH, cilt.38, sa.1, ss.19-28, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1177/07482337211054372
  • Dergi Adı: TOXICOLOGY AND INDUSTRIAL HEALTH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, BIOSIS, Communication Abstracts, EMBASE, Environment Index, Food Science & Technology Abstracts, Index Islamicus, MEDLINE, Metadex, Pollution Abstracts, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.19-28
  • Anahtar Kelimeler: Bisphenol A, obesogen, fibroblast growth factor, zebrafish, inflammatory cytokines, OBESITY, EXPRESSION, MECHANISMS, LEPTIN, RESISTANCE, BPA
  • Marmara Üniversitesi Adresli: Evet

Özet

Obesogens affect lipid metabolism, and genetic or epigenetic factors may also contribute to the progression of obesity. Endocrine-disrupting chemicals (EDCs) are the most striking among obesogens. Bisphenol A (BPA) is an estrogenic EDC used in food containers, adhesives, dye powders, and dental fillers. We aimed to elucidate molecular mechanisms of BPA's obesogenic effects focusing on obesogenic pathways in the liver including fibroblast growth factor (FGF) and Dnmt3a which is its epigenetic regulator, oxidant-antioxidant status, and inflammatory cytokines. Zebrafish were divided into three groups as control, low-dose BPA (1 mu m BPA), and high-dose BPA groups (10 mu m BPA). At the end of 30 days, oral glucose tolerance test (OGTT) was performed, fasting blood glucose levels were measured, and hepatopancreas tissues were taken. Malondialdehyde (MDA) levels, superoxide dismutase (SOD), glutathione S-transferase (GST), and nitric oxide (NO) activities were examined in the hepatopancreas. Inflammatory cytokines, lepa, fgf21, and dnmt3a expressions were determined by RT-PCR. BPA exposure increased the body weights, il1ss, tnf alpha, il6, lepa, fgf21, and dnmt3a expressions, impaired glucose tolerance, and oxidant-antioxidant status in a dose-dependent manner. Hepatocyte degeneration, lipid vacuolization, and vasocongestion were observed in both BPA-exposed groups. Our study suggests impaired glucose tolerance, oxidant-antioxidant balance, increased inflammatory response, fgf21 expression, and dnmt3a expressions as the possible mechanisms for the BPA-induced obesity model in zebrafish.