Community changes during start-up in methanogenic bioreactors exposed to increasing levels of ammonia

Calli B., Mertoglu B., Inanc B., Yenigun O.

ENVIRONMENTAL TECHNOLOGY, cilt.26, sa.1, ss.85-91, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 1
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1080/09593332608618585
  • Dergi Adı: ENVIRONMENTAL TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.85-91
  • Anahtar Kelimeler: DGGE, FISH, free ammonia, Methanosaeta, Methanosarcina, ANAEROBIC-DIGESTION, MOISTURE-CONTENT, SLUDGE, INHIBITION, DIVERSITY, LANDFILL, REACTORS, WASTE, PH
  • Marmara Üniversitesi Adresli: Evet

Özet

To investigate the methanogenic population dynamics during the start-up under gradually increasing free ammonia levels, five mesophilic UASB reactors seeded with different sludges were operated for 140 days. Changes in the methanogenic community investigated by using 16S rDNA/rRNA based molecular methods such as denaturing gradient gel electrophoresis (DGGE), fluorescent in-situ hybridization (FISH), cloning and DNA sequencing. Free ammonia nitrogen (FAN) in the reactors was gradually increased from 50 to 130 mg l(-1) by increasing total ammonia nitrogen (TAN) from 1000 to 2500 mg l(-1) in the feed and keeping reactor pH at 7.7 in the active zone. Even at highest FAN level, COD and VFA removal efficiencies above 90 and 98% were obtained, respectively. However, Methanosaeta-related species mainly prevailing in seed sludges were substituted for Methanosarcina as the abundant methanogens although reactor performances were almost comparable and constant.