Akademik Veri Yönetim Sistemi
European Journal of Research in Dentistry, 2025 (Hakemli Dergi)
Objectives: This in vitro study aimed to evaluate the effects of levan-based hydrogels recognized for their anti-inflammatory properties on the viability of dental pulp-derived mesenchymal stem cells (DPSCs), and to compare them with two commonly used pulp capping materials: mineral trioxide aggregate (MTA) and Biodentine.
Materials and Methods: Enzymatic levan (EL) hydrogel was synthesized using the levansucrase enzyme. A second formulation, EL/ nanohydroxyapatite (nHAP), was prepared by incorporating nHAP in a 1:1 ratio. MTA, Biodentine, and both hydrogel formulations were incubated in Dulbecco's Modified Eagle Medium (DMEM) for 3 days to generate extracts. DPSCs were seeded in 96-well plates at a density of 100,000 cells per plate. The extracts were applied to the cells, and viability was assessed at 24, 48, and 120 hours using the MTT assay. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test (p<0.05).
Results: Levan-based hydrogels showed enhanced cell viability at 48 hours (mean: 123.4), suggesting short-term biocompatibility. However, by 120 hours, cell viability significantly declined (mean: 22.0). In contrast, MTA exhibited a gradual and sustained increase in viability over the same period, reaching a mean of 219.8 at 120 hours. Biodentine presented intermediate effects between the hydrogels and MTA.
Conclusions: Levan-based hydrogels demonstrated promising short-term biocompatibility with DPSCs. However, MTA may provide a more stable environment conducive to long-term cell viability. These findings support further investigation into the optimization of levan-based formulations for pulp capping applications.