Akademik Veri Yönetim Sistemi
Biologia, cilt.81, sa.1, 2026 (SCI-Expanded, Scopus)
Probiotic bacteria are widely used due to their health benefits and generally recognized as safe (GRAS) status. With increasing consumer demand, novel probiotic strains have gained attention. In this study, fifteen Gram-positive strains were isolated from traditional Ezine cheese and evaluated for probiotic potential. These fifteen Gram-positive strains were evaluated extracellular enzyme activities, antibiotic resistance and safety parameters. The bioactivity and probiotic properties of four selected strains (E5, E6, J2 and J5), which were thought to have probiotic potential, were then tested. The isolates were identified by 16S rRNA sequencing as Lactobacillus paracasei, Lactococcus garvieae, and Priestia flexa with over 99.89% similarity. While antibacterial activity of CFS samples of these isolates against Bacillus cereus and Staphylococcus aureus was not detected, their antibiofilm activities were significant. CFS samples of E5 and E6 exhibited over 50% inhibition against S. aureus biofilms whereas CFS samples of isolates E5, E6 and J2 exhibited antibiofilm effect over 80%. Antibiotic susceptibility tests revealed that E5, E6, and J2 were resistant to tested antibiotics, whereas J5 was susceptible to ciprofloxacin and tetracycline, and moderately susceptible to kanamycin. None of the isolates exhibited hemolytic or gelatinase activity, and all showed high survival rates under bile salt stress. Moreover, the isolates demonstrated high autoaggregation and coaggregation abilities, suggesting a potential to inhibit foodborne pathogen colonization. These findings suggest that isolates E5, E6, J2, and J5 possess promising probiotic properties and could be considered as potential candidates for future applications in food or health-related industries.