© This is an Open Access article distributed under the terms of the Creative Commons Attribution NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Background: Health information technologies (HITs) present numerous opportunities for the improvement and transformation of healthcare, which include reducing human errors, improving clinical outcomes, facilitating care coordination, improving efficiency of practice and tracking data over time. HITs involve various technologies that range from simple charting, to a more advanced decision support and integration with medical technology. Objective: The aims of this study were to examine the readiness for the implementation of health information technologies (HITs) among medical and administrative staff as well as to evaluate the effects of information security status on the readiness. Methods: In this cross-sectional study, 236 medical employees (F/M: 192/44; mean age: 34±7.43 years) and 139 administrative employees (F/M: 93/46, mean age: 36±7.64 years) from 15 public health institutions in Kocaeli, Marmara Region were included. The data were collected via a structured questionnaire regarding opinions about information security and privacy, use of information technologies and the Organizational Information Technology Innovation Readiness Scale (OITIRS). After an explanatory factor analysis was performed for the scale, two subgroups regarding Organizational Readiness and Technological Readiness were obtained. Binary logistic regression analyses were performed to evaluate related factors for these subgroups of OITIRS. Results: According to binary logistic regression analysis, establishing of a password management system was found to be a crucial factor for both organizational and technological readiness among medical and administrative employees in health institutions (p<0.05). The enhancement of collaboration among staff by implementing information technologies was a critical factor for the medical staff; whereas, the attitude of employees to ensure information security was an important factor for the administrative employees in both subgroups (p<0.05). Conclusion: Both medical and administrative unit employees stated that establishing a password management system that determines the frequency of changing passwords in the organization would affect both organizational and technical readiness in healthcare institutions.