Men's jacket is a garment made by combining fabrics, interlining and lining materials. These materials have their own thermal comfort features. In this work, the thermal comfort properties of the materials forming the jacket are investigated as mono layer and multilayer. The materials were tested as a single layer in first configuration. Then interlinings were fixed to fabrics and tests have been carried out as two layers. Finally, fabrics, interlinings and linings were tested as triple layer. According to the test results, the increase in the number of layers causes the decrease in air and water vapour permeability, and causes increase in the thermal resistance of the material.