Assessment of poly(3-hydroxybutyrate) synthesis from a novel obligate alkaliphilic Bacillus marmarensis and generation of its composite scaffold via electrospinning
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.119, ss.982-991, 2018 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 119
- Basım Tarihi: 2018
- Doi Numarası: 10.1016/j.ijbiomac.2018.08.014
- Dergi Adı: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.982-991
- Anahtar Kelimeler: Bacillus marmarensis, Poly(3-hydroxybutyrate), Composite nanofiber scaffold, TISSUE ENGINEERING APPLICATIONS, POLY-BETA-HYDROXYBUTYRATE, POLYHYDROXYBUTYRATE PRODUCTION, PHB, CEREUS, POLYHYDROXYALKANOATES, NANOPARTICLES, NANOFIBERS, POLY-3-HYDROXYBUTYRATE, BIOSYNTHESIS
- Marmara Üniversitesi Adresli: Evet
Özet
In this study, poly(3-hydroxybutyrate) (PHB) production from a newly isolated obligate alkaliphilic Bacillus marmarensis DSM 21297 was investigated to evaluate the ability of obligate alkaliphilic strain to produce a biopolymer. Additionally, electrospun nanofibers from B. marmarensis PHB (Bm-PHB) were generated using Bm-PHB/polycaprolactone (PCL) blend to evaluate the applicability of Bm-PHB. According to the experimental results, the metabolic activity of B. marmarensis decreased the pH of the medium by generating H+ ions to initiate Bm-PHB production, which was achieved at pH below 9.0. Regarding medium components, the addition of MgSO4 center dot 7H(2)O and KH2PO4 to the medium containing 1% glucose enhanced the amount of Bm-PHB synthesis, and an approximately 60% increase in PHB concentration was obtained in the presence of mineral salts.