Assessment of poly(3-hydroxybutyrate) synthesis from a novel obligate alkaliphilic Bacillus marmarensis and generation of its composite scaffold via electrospinning

ÖZGÖREN CAN T., PİNAR O., Bozdag G., Denizci A. A., GÜNDÜZ O., Hatir P. C., ...Daha Fazla

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.119, ss.982-991, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 119
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1016/j.ijbiomac.2018.08.014
  • Dergi Adı: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.982-991
  • Anahtar Kelimeler: Bacillus marmarensis, Poly(3-hydroxybutyrate), Composite nanofiber scaffold, TISSUE ENGINEERING APPLICATIONS, POLY-BETA-HYDROXYBUTYRATE, POLYHYDROXYBUTYRATE PRODUCTION, PHB, CEREUS, POLYHYDROXYALKANOATES, NANOPARTICLES, NANOFIBERS, POLY-3-HYDROXYBUTYRATE, BIOSYNTHESIS
  • Marmara Üniversitesi Adresli: Evet

Özet

In this study, poly(3-hydroxybutyrate) (PHB) production from a newly isolated obligate alkaliphilic Bacillus marmarensis DSM 21297 was investigated to evaluate the ability of obligate alkaliphilic strain to produce a biopolymer. Additionally, electrospun nanofibers from B. marmarensis PHB (Bm-PHB) were generated using Bm-PHB/polycaprolactone (PCL) blend to evaluate the applicability of Bm-PHB. According to the experimental results, the metabolic activity of B. marmarensis decreased the pH of the medium by generating H+ ions to initiate Bm-PHB production, which was achieved at pH below 9.0. Regarding medium components, the addition of MgSO4 center dot 7H(2)O and KH2PO4 to the medium containing 1% glucose enhanced the amount of Bm-PHB synthesis, and an approximately 60% increase in PHB concentration was obtained in the presence of mineral salts.