A total of 16 wild species of the genus Lathyrus grown wildly in the region of Thrace (Turkey) were collected, identified and examined for karyotype analysis. The species diversity found in this work includes approximately one fourth of all the Lathyrus species in Turkey. Collected specimen were identified as L. niger (L.)Bernh. subsp. niger, L. palustris L. subsp. palustris, L. laxiflorus (Desf.) O.Kuntze subsp. laxiflorus, L. tuberosus L., L. undulatus Boiss., L. sylvestris L., L. sphaericus Retz., L. setifolius L., L. annuus L., L gorgoni Parl. var. pilosus C.C.Townsend, L. cicera L., L. hirsutus L., L. clymenum L., L. ochrus (L.)DC., L. nissolia L., L. aphaca L. var. aphaca. The chromosome number of A studied taxa except L. palustris L. subsp. palustris (2n=42) was determined 2n=14, x=7.