MolBioKon 2019, İstanbul, Türkiye, 27 - 29 Eylül 2019
K562 cells are used to as model systems for normal myeloid, development of myeloid leukemia, control of differentiation studies. K562 cell line represent early differentiation stage in granulacyte leneage. Non-neuronal acetylcholine have a important role in migration, proliferation and differentiation of different types of cell. It was recently suggested that the non-neuronal cholinergic system has also a important role in different types of cancer cells. Muscarinic receptors are part of G protein coupled receptors . K562 cells express M2,M3 and M4 muscarinic receptors. Especially, muscarinic type 3 receptor (M3R) plays a important role in different cancer cells. Bax and Bcl-2 proteins are related to apoptosis.
Aim of this study firstly was to examine effects of pilocarpine, TNFalpha and other drugs on apoptosis. Secondly, we aimed to demonstrate the mitogenenic effects of FBS, TNF alpha and EGF in K562 cells proliferation. Effects of Pilocarpin on The AChE activity has been detected.
Materials and methods
Cell viability were evaluated by the 0.4% trypan blue exclusion test by using a hemocytometer. Proliferation assays were performed by measuring BrdU incorporation during DNA synthesis in proliferating cells. Effects of TNF alpha, EGF, M3 agonist and antagonist on bcl and bax expression were detected by western blotting method. AChE level was detected by the QuantiChrom Acetylcholinesterase Assay Kit (DACE-100).
This study, proposes that pilocarpine mediated M3R activation can promote apoptosis and inhibit cell proliferation