Development and Programmed Cell Death in the Filament Cells of Lathyrus undulatus Boiss.

Vardar F., Ünal M.

Caryologia, cilt.64, sa.2, ss.164-172, 2011 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 64 Sayı: 2
  • Basım Tarihi: 2011
  • Dergi Adı: Caryologia
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.164-172
  • Marmara Üniversitesi Adresli: Evet

Özet

The androecium in Lathyrus undulatus Boiss. consisted of 10 stamens. The anther was joined to the

fi lament on the outward facing surface (dorsifi xed). After dehiscence anther and fi lament separate from connecting

edge and anther falls off. In the present study development and programmed cell death process in the

fi lament cells of Lathyrus undulatus Boiss. (Fabaceae) were analyzed by light, fl uorescence and electron microcopy,

during pollen development. The observations indicated that fi lament cells most fully developed at tetrad

stage. The cytochemical identifi cations of proteins, insoluble polysaccharides and lipids within and around the

vascular bundle suggest that fi lament cells are involved in the transport of the organic materials. Moreover,

fi lament cells subject to programmed cell death confi rmed with TUNEL from young to mature pollen stage

including structural alterations such as nucleus and chromatin condensation, vacuolization, concentric circle

of membranes and disintegrated plastids. Granular materials and electron lucent areas in the cytoplasm were

common at mature pollen stage. The attained data suggests that degenerated anther separate from fi lament by

programmed cell death of the connecting edge.