Effect of extremely low frequency electromagnetic fields on bacterial membrane

ÖNCÜL Ş., Cuce E. M., Aksu B., Garip A. I.

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, cilt.92, sa.1, ss.42-49, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 92 Sayı: 1
  • Basım Tarihi: 2016
  • Doi Numarası: 10.3109/09553002.2015.1101500
  • Dergi Adı: INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.42-49
  • Anahtar Kelimeler: ELF-EMF, bacterial membrane potential, surface potential, bacterial hydrophobicity, respiratory activity, bacterial growth, ESCHERICHIA-COLI-CELLS, ELF MAGNETIC-FIELDS, BIOSYNTHETIC REQUIREMENTS, ELECTRIC-FIELDS, EXPOSURE, SURFACE, INHIBITION, REPAIR, DAMAGE, GROEL
  • Marmara Üniversitesi Adresli: Evet

Özet

Purpose: The effect of extremely low frequency electromagnetic fields (ELF-EMF) on bacteria has attracted attention due to its potential for beneficial uses. This research aimed to determine the effect of ELF-EMF on bacterial membrane namely the membrane potential, surface potential, hydrophobicity, respiratory activity and growth.Materials and methods: Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli were subjected to ELF-EMF, 50Hz, 1mT for 2h. Membrane potential was determined by fluorescence spectroscopy with or without EDTA (Ethylenediaminetetraacetic acid) with DisC3(5) (3,3-dipropylthiacarbocyanine iodide), zeta potential measurements were performed by electrophoretic mobility, hydrophobicity of the membrane was measured with MATH (Microbial Adhesion to Hydrocarbons) test, respiratory activity was determined with CTC (5-Cyano-2,3-ditolyl tetrazolium chloride), colony forming unit (CFU) and DAPI (4,6-diamidino-2-phenylindole, dihydrochloride) was used for growth determinations.Results: ELF-EMF caused changes in physicochemical properties of both Gram-positive and Gram-negative bacteria. Hyperpolarization was seen in S. aureus and EDTA-treated E. coli. Surface potential showed a positive shift in S. aureus contrariwise to the negative shift seen in EDTA-untreated E. coli. Respiratory activity increased in both bacteria. A slight decrease in growth was observed.Conclusion: These results show that ELF-EMF affects the crucial physicochemical processes in both Gram-positive and Gram-negative bacteria which need further research.