HIV-1 viral proteins gp120 and Tat induce oxidative stress in brain endothelial cells

Price T., Ercal N., Nakaoke R., Banks W.

BRAIN RESEARCH, cilt.1045, ss.57-63, 2005 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1045
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/j.brainres.2005.03.031
  • Dergi Adı: BRAIN RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.57-63
  • Anahtar Kelimeler: blood-brain barrier, oxidative stress, gp120 protein, Tat protein, HIV-1, dementia, IN-VITRO MODEL, NERVOUS-SYSTEM, BARRIER, PERMEABILITY, GLUTATHIONE, INTEGRITY, MIGRATION, PROTECTS, DAMAGE, VIVO
  • Marmara Üniversitesi Adresli: Hayır

Özet

The blood-brain barrier (BBB) has an important role in the development of AIDS dementia. The HIV-1 envelope glycoprotein (gp120) and transregulatory protein (Tat) of HIV-1 are neurotoxic and cytotoxic and have been implicated in the development of HIV dementia. They are known to cause oxidative stress and are associated with disruption of the BBB. Here, we used an immortalized endothelial cell line from rat brain capillaries, RBE4, to determine whether gp 120 and Tat can induce oxidative stress in an in vitro model of the BBB. RBE4 cells were exposed to gp120 or Tat and the levels of reduced glutathione (GSH), oxidized glutathione (GSSG), catalase (CAT) activity, glutathione peroxidase (GPx) activity, and glutathione reductase (GR) activity, and malondialdehyde (MDA) used as measures of oxidative stress. Both gp120 and Tat significantly decreased the levels of intracellular GSH, GPx, and GR and increased the levels of NIDA in RBE4 cells, showing that the cells were oxidatively challenged. The ratio of GSH/GSSG, a widely accepted indicator of oxidative stress, was also significantly decreased. These studies show that both of these viral proteins can induce oxidative stress in immortalized BBB endothelial cells. (C) 2005 Elsevier B.V. All rights reserved.