A novel approach for rapid screening of mitochondrial D310 polymorphism


Aral C., Kaya H., Ataizi-Celikel C., Akkiprik M. , Sonmez O., Gulluoglu B. , ...Daha Fazla

BMC CANCER, cilt.6, 2006 (SCI İndekslerine Giren Dergi) identifier identifier identifier

Özet

Background: Mutations in the mitochondrial DNA ( mtDNA) have been reported in a wide variety of human neoplasms. A polynucleotide tract extending from 303 to 315 nucleotide positions (D310) within the non-coding region of mtDNA has been identified as a mutational hotspot of primary tumors. This region consists of two polycytosine stretches interrupted by a thymidine nucleotide. The number of cytosines at the first and second stretches are 7 and 5 respectively, according to the GeneBank sequence. The first stretch exhibits a polymorphic length variation (6-C to 9-C) among individuals and has been investigated in many cancer types. Large-scale studies are needed to clarify the relationship between cytosine number and cancer development/progression. However, time and money consuming methods such as radioactivity-based gel electrophoresis and sequencing, are not appropriate for the determination of this polymorphism for large case-control studies. In this study, we conducted a rapid RFLP analysis using a restriction enzyme, BsaXI, for the single step simple determination of 7-C carriers at the first stretch in D310 region.