Fluoride, cilt.59, sa.3, 2026 (SCI-Expanded, Scopus)
Purpose: This in vitro study aimed to evaluate the microleakage and bond interface morphology of two different fissure sealants applied after silver diamine fluoride (SDF) treatment with varying application times. Methods: A total of 72 extracted permanent molars were randomly divided into six groups (n=12). Half of the samples were restored with a glass ionomer fissure sealant (Fuji Triage, GC Corporation, Japan), and the other half with a resin-based fissure sealant (Clinpro Sealant, 3M ESPE, USA). Among the six groups, two served as controls with no SDF application. In two groups, 38% silver diamine fluoride (SDF) was applied and waited for 5 minutes prior to sealant placement. In the remaining two groups, SDF application was applied and waited for one week before the sealant procedure. Microleakage was assessed using the dye penetration method after thermocycling, and the bond interface was evaluated by scanning electron microscopy (SEM). Microleakage scores were evaluated under a stereomicroscope according to Williams and Winter scoring criteria. SEM images were analyzed at magnifications of 500x, and 4000x. Statistical analysis was conducted using the Kruskal–Wallis test with Dunn’s post-hoc comparisons. Results: Glass ionomer fissure sealant exhibited significantly lower microleakage scores compared to resin-based fissure sealant (p <0.001). Microleakage was reduced when a 1-week waiting period was applied after SDF treatment. In terms of bond interface, resin-based fissure sealant demonstrated shorter bonding lengths, indicating a stronger bond compared to Glass ionomer fissure sealant (p < 0.001). Conclusions: The type of fissure sealant and timing of SDF application significantly influence microleakage and bond strength. Glass ionomer fissure sealant is more favorable for minimizing microleakage, whereas resin-based fissure sealant exhibits superior bonding characteristics. Clinical application should consider these factors to optimize sealant performance.