A flow cytometric method of ligand binding to isolated receptors using micro beads


Tetik S., Yardimci K., Eksioglu-Demiralp E.

17th European and African Division Meeting of the International-Society-of-Haematology, Graz, Avusturya, 7 - 10 Eylül 2003, ss.215-218 identifier

  • Basıldığı Şehir: Graz
  • Basıldığı Ülke: Avusturya
  • Sayfa Sayısı: ss.215-218

Özet

There are various methods in measuring the ligand binding to receptors. A quantitative flow cytometric method of described for binding measurements of FITC labelled ligands to receptor coated micro beads. GpIIb/IIIa complex was purified from human platelets by affinity chromatography and coated to polystyrene micro beads. Fibrinogen (Fg) was labelled with FITC. Different concentrations of Fg-FITC with GpIIb/IIIa coated micro beads were incubated and analyzed by flow cytometry. BSA-FITC was used as a negative control. The Fg-FITC binding to isolated platelets was also studied under the same conditions. This method besides showing quantitative ligand binding to the receptor, it also reflects the in vivo binding conditions.