Akademik Veri Yönetim Sistemi
Journal of the Indian Chemical Society, cilt.103, sa.1, 2026 (SCI-Expanded, Scopus)
In this study, four different polyconjugated aromatic imines were synthesized using ortho– and para– diamines with and without hydroxyl groups in order to control bacterial biofilm formation at molecular-level through the intercalation of bacterial second messenger, cyclic dimeric guanosine 3′,5′-monophosphate (c-di-GMP). As key regulators of biofilm formation and several vital functions of bacteria, c-di-GMP molecules were extracted and c-di-GMP intercalation capabilities of four different polyconjugated imines were compared by monitoring UV absorbance reductions and fluorescence emission quenching. The results showed that the compounds without hydroxyl groups reached a 66 % UV absorbance reduction. Moreover, aza-BODIPY was tested as a novel c-di-GMP intercalator, showing up to 45 % reduction on UV absorbance and up to 40 % quenching on fluorescence emission suggesting its potential for bacterial control. Biofilm formation/inhibition studies on a 96-well microtiter dish confirmed the obtained results. Intercalation studies revealed that the selected ligands effectively interacted with c-di-GMP, suggesting their potential to modulate bacterial signalling pathways. These findings offer a promising strategy for controlling biofilm forming pathogens by interfering with their biofilm regulatory mechanisms.