Essential oil composition of Centaurea stenolepis Kerner flowers and stems were investigated with GC, GC-MS. Flowers and stems of C. stenolepis had very low essential oil yield <0.01 % (v/w). Twenty eight compounds were identified in the flower oil which represent 53.5 % of the oil. Main components of the flower oil include caryophyllene oxide 12.6 %, hexadecanoic acid 10.6 % and beta-Eudesmol 7.2 %. Forty compounds were identified in the stem oils which represent 74.2 % of the oil. Main components of the stem oils include hexadecanoic acid 38.4 % and phytol 12.9 %. Both flower and stem oils were rich on oxygenated sesquiterpene hydrocarbons and fatty acids.