Overexpression of miR-145-5p Inhibits Proliferation of Prostate Cancer Cells and Reduces SOX2 Expression

ÖZEN M., Karatas O. F., GÜLLÜOĞLU Ş., Bayrak O. F., Sevli S., Guzel E., ...Daha Fazla

CANCER INVESTIGATION, cilt.33, sa.6, ss.251-258, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33 Sayı: 6
  • Basım Tarihi: 2015
  • Doi Numarası: 10.3109/07357907.2015.1025407
  • Dergi Adı: CANCER INVESTIGATION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.251-258
  • Anahtar Kelimeler: hsa-miR-145-5p, SOX2, Prostate cancer, Stem cell marker, Cancer stem cell, MICRORNA EXPRESSION, TARGETS, GENES, DATABASE
  • Marmara Üniversitesi Adresli: Hayır

Özet

We aimed to perform functional analysis of miR-145-5p in prostate cancer (PCa) cells and to identify targets of miR-145-5p for understanding its role in PCa pathogenesis. PC3, DU145, LNCaP PCa, and PNT1a nontumorigenic prostate cell lines were utilized for functional analysis of miR-145-5p. Its overexpression caused inhibition of proliferation through apoptosis and reduced migration in PCa cells. SOX2 expression was significantly decreased in both mRNA and protein level in miR-145-5p-overexpressed PCa cells. We proposed that miR-145-5p, being an important regulator of SOX2, carries a crucial role in PCa tumorigenesis.