The comparison of miRNA expression levels in Type 2 diabetes and diabetic peripheral neuropathy patients

Ayaz A., Kuras S., Erdogan B., Aktas H. S., Pence M. E., Pence H. H.

Clinical Biochemistry, cilt.138, 2025 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 138
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1016/j.clinbiochem.2025.110946
  • Dergi Adı: Clinical Biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, EMBASE, Veterinary Science Database
  • Anahtar Kelimeler: Diabetic neuropathy, miR-375, Type 2 diabetes
  • Marmara Üniversitesi Adresli: Hayır

Özet

Objectives: We aimed to investigate the correlation between miRNA expression levels in patients with type 2 diabetes mellitus (T2DM) and diabetic peripheral neuropathy (DPN) and determine their potential functions in DPN. Material and Methods: The study participants were between 30 and 75 years old and divided into three groups: 30 patients with Type 2 T2DM without DPN (group 1), 30 patients with DPN (group 2), and 30 healthy controls (group 3). Whole blood samples were obtained from the individuals forming the groups, and the expression of the miR-128a, miR-146a, miR-155, and miR-375 genes in these samples was determined by real-time polymerase chain reaction (PCR). RNU6 was used as the housekeeping gene. Results: No significant differences were observed in the expression levels of miR-128a, miR-146a, and miR-155 among all groups (p > 0.05). However, miR-375 expression levels differed significantly between the group 2 and the group 3 (p < 0.05). No significant differences in miR-375 expression levels were found between the group 1 and the group 3, nor between the group 1 and the group 2 (p > 0.05). Conclusions: miR-375 may be linked to the progression of diabetic peripheral neuropathy (DPN).